IMMUNOMICS SERVICES
Protein and Peptide (Auto-)Antibody Reactivity Profiling
Overview
AIT offers a unique and comprehensive technology portfolio for in-depth immune system analysis. We drive translational research in immunology, virology, autoimmunity, cancerous/systemic, allergic and infectious disease, advancing the discovery of novel biomarkers and insights into immune responses.
AIT offers cutting-edge, high-throughput technologies for antibody profiling using protein and peptide microarrays, bead-based multiplex assays, ELISA, and NGS-based platforms. These services—both standardized and custom-developed—enable the detection of immunoglobulin patterns in various body fluids, which serve as early diagnostic biomarkers for autoimmune, inflammatory, aging-related, and cancerous diseases. Advanced array platforms include KREX™ protein arrays (with correctly folded proteins) and CDI proteome arrays, alongside NGS based approaches like PhIP-Seq and MIPSA technologies for in-depth immune reactivity analysis. All services are supported by robust bioinformatics tools to deliver comprehensive immunoassay and biomarker solutions for research and diagnostics.
Applications
Immunomics & Antibody Profiling: Comprehensive analysis of antibody reactivity patterns using advanced array and sequencing platforms.
Biomarker Discovery & Epitope Mapping: Identification of disease-relevant biomarkers and epitopes selecting from broadcast spectrum of technologies.
Therapeutic Monitoring: Evaluation of immune responses and detection of off-target reactivity in treated patient samples, particularly in vaccine development.
Diagnostics & Vaccine Development: Definition of peptide candidates for diagnostic and vaccine applications across a broad spectrum of diseases, including infections, allergies, autoimmunity, and systemic conditions.
Companion Diagnostics: Detection of unintended immune responses and support for targeted therapeutic strategies through off-target reactivity profiling.
Our core Expertise
- High-density protein and peptide microarrays
- Multiplex protein analysis using bead-arrays and ELISA
- NGS-based antibody profiling (PhIP-Seq, MIPSA)
- Custom assay development and validation
- Data analysis with integrated bioinformatics support
Immunomics platforms & Technologies
Protein Microarray and Beadarray Services
Click below to explore available assay platforms:
Product / Assay | Description |
AIT-16k Human Protein Array | Discover (auto)antibody profiles using 10µl serum or plasma – on AIT’s 16k protein-array resembling 6.129 different human proteins covering 8.173 annotated human transcripts derived from 15.286 recombinant E. coli expression clones |
AIT-260x Pathogen Array | AIT’s 260x human pathogen array – antibody profiling associated with bacterial infections |
Customized Protein & Peptide Microarrays(100-32,000 features) | Inquire for custom protein- and peptide microarray generation and services – ideal for >100-30000 features – several formats of e.g. 24 subarray per slide with e.g. 600 different peptides/proteins per subarray) available – ideal for high multiplexed and moderate to high sample throughput |
Customized Protein & Peptide Bed-Arrays(5-500 plexed) | Ideal for 5-500 plexed and high sample numbers – serotesting as well other immuno-assays (but also DNA or RNA assays) available |
Product / Assay | Description |
i-Ome™ Discovery | 1,800-plus proteins spanning numerous functions and disease areas |
i-Ome Cancer | 500-plus proteins associated with cancer, including therapeutic targets, cancer-testis antigens and more |
Pan-Autoimmune | 500-plus proteins clinically relevant in autoimmune diseases |
OncoREX p53 | 100-plus p53 wildtype and mutant variants for antibody and protein binding screens |
Product / Assay | Description |
Proteome Wide Autoantibody Discovery and Profiling | HuProt™ Human Proteome Microarray with >21,000 full-length, folded 3D proteins for conformational binding |
HuProt™ and MouseScan™ for epitope-level whole proteome profiling using Phage ImmunoPrecipitation Sequencing (PhIP-Seq) | |
High-throughput Focused/Custom microarrays available for biomarker validation experiments | |
Target ID Services | Understand interactions with the human proteome using the HuProt™ Human Proteome Microarray with >21,000 full-length, folded 3D proteins for conformational binding. |
Applications: Protein-Protein /DNA interactions, Antibody specificity validation, Target validation, etc. | |
Viral Exposure Profiling | VirScan® uses Phage ImmunoPrecipitation Sequencing (PhIP-Seq) identify an individual’s unique history of viral exposures. |
Applications include: Pathogen surveillance. | |
The study of the relationships between viral infection and chronic disease – identifying potential disease triggers; | |
Understanding host-pathogen interactions and their impact on the immune system. |
NGS-Based Antibody Profiling
Explore high-resolution immune profiling technologies based on phage display and next-generation sequencing.
Infinity Bio – MIPSA Technology
MIPSA technology to provide unprecedented insights into antibody reactivity for translational research and therapeutic development.
- MIPSA antibody reactome profiling, offers unparalleled depth in immune system analysis, advancing immunology, virology, autoimmunity, and allergy research, enabling the discovery of new biomarkers and providing disease insights.
- Proprietary MIPSA technology (Molecular Indexing of Proteins by Self-Assembling) is a leap forward in antibody profiling. It achieves unmatched breadth and resolution in mapping the molecular targets of immune responses. The current catalog of antigen panels – VirSIGHT, HuSIGHT, MuSIGHT and EnviroSIGHT – blend full-length protein and peptide libraries, providing the most in-depth assays for detecting antibodies targeting all human viruses, self-proteins, or allergic proteins, respectively, all in a single test.
Product / Assay | Description |
HuSIGHT | Whole human proteome antigenic profiling using a comprehensive peptide library designed to represent all human proteinsScope: Covers 27,703 unique genes;Includes 385,432 unique protein isoforms (each isoform tiled by peptides). |
MuSIGHT ™ | Comprehensive peptide library designed to represent all mouse proteins |
VirSIGHT™ | Human virome antibody reactome profiler, designed to provide a comprehensive overview of an individual’s viral exposure and vaccine response history. Key features include: |
EnviroSIGHT™ | EnviroSIGHT™ enables high-resolution profiling of antibody responses to a broad spectrum of allergens, non-viral pathogens and microbial antigens from diverse sources. It opens up an unprecedented window into how environmental exposures—from everyday allergens and the microbiome to pathogenic bacteria and parasites—sculpt our adaptive immune system. This detailed insight is crucial for unlocking new knowledge in both infectious and chronic disease pathways. Key features include:Scope: The most comprehensive peptide-based library available to environmental antigens – Includes 3,437 species-level taxonomic IDs. |
PhIP-Seq Services
- Customised generation of phage-display libraries for presentation of e.g. up to 50aa long peptides on M13 a/o T7 phages
- Conduct enrichment and differential antigenic reactivity analysis of sera/plasma samples or for epitope and antibody-specificity testing e.g. detecting antibodies targeting otherwise not accessible peptides
- Epitope mapping and specificity screening.
RESEARCH SERVICES AND CAPABILITIES
- Recombinant protein production and purification
- Custom array generation and sample analysis (microarray and bead-based)
- Assay development using planar and bead array plattforms
- Bioinformatics, statistical analysis and reporting
- Consulting and experimental design support
Other protein based services
- Analyses services using “catalogue assays” (Luminex® and ELISA)
- Custom assay design and development
- Targeted PROTEOMICS using OLINK® Proximity Extension Assay (PEA) Technology – https://molecular-diagnostics.ait.ac.at/services/olink/
New
Use your clinical samples for non-consuming measurement of metabolomic & lipoprotein profiling – by our partner lifespin – we will arrange sample measurement in advance of subsequent analyses conducted at our lab (exosomal miRNA, cfRNA, cfDNA, proteomics, immunomics).
