High-throughput proteomics
AIT has long lasting experience with the OLINK® Proximity Extension Assay (PEA) – technology for targeted high-throughput proteomics analysis and acts as a certified service provider, supporting customers from experimental planning & study design to pre-analytics processing and standardisation, sample analysis, bioinformatic data analysis, visualisation and interpretation. A broad range of assay formats enables your proteomics analysis at different scale for several sample types and various applications.
Having a broad technology portfolio including OLINK® technology, AIT-Molecular Diagnostics offers also multi-omics analysis.
AIT is ISO9001 certified and have been qualified by OLINK to provide services based on Olink® technology.
Image(AIT): Olink assays for targeted proteomics analysis
OLINK® Proximity Extension Assay (PEA) - technology
OLINK® PEA technology is based on the detection of biomarkers generated by binding specific pairs of DNA-tagged antibodies. Matched DNA-tags form hybridization products, and Proximity Extension Assay (PEA) technology forms reporter-templates for multiplexed PCR amplification enabling high-throughput detection of the DNA-reporter-sequences by qPCR or NGS. Due to PCR amplification, this technology allows parallel quantification of many markers over a wide range of several orders of magnitudes, although when biomarkers are present at very different levels in the original specimen. Thus relative or absolute quantification of a multitude of biomarkers can be performed from minute amounts of sample volume in a very efficient manner.
See: https://www.olink.com/our-platform/our-pea-technology/
OLINK® key features
- high-throughput proteomics compatible with different sample types
- low sample consumption
- high specificity due to use of of target specific antibodies pairs
- high sensitivity & wide dynamic range – due to PCR-based or NGS readout
- optimised biomarker detection range according to human serum & plasma
- broad range of clinically and biologically relevant panels available for discovery and / or confirmation – and customised flex panels for e.g. validation
Applications
OLINK® Proximity Extension Assay (PEA) -technology provides powerful proteomics analysis. The power of this technology and the broad variety of applications are reflected be a stunning high and rapidly growing number of scientific publications – by 2026 over 3600+ scientific publications.
- protein biomarker studies, – diagnostic, prognostic, predictive and stratification
- treatment response studies
- multi-omics – ideal & simple supplement in combination with other omics-studies
- understanding human biology
- drug discovery and development
- …and many more applications and settings – see relevant publications & whitepapers
SAMPLE TYPES
Human plasma and serum are the recommended sample types for the use of all Olink panels. EDTA (ethylenediaminetetraacetic acid) plasma is preferred, but citrate and heparin plasma are accepted as well. Furthermore, Olink technology is compatible with many other matrices e.g. a multitude of bodily fluids (e.g. cerebrospinal fluid-CSF, saliva, urine, etc.), conditioned media, tissue lysates, dried blood spot-DBS and many more. Please note that alternative matrices might require specialized processing and pre-processing. For further information please inquire about your specific project setting.
OLINK® formats provided
Olink Target 96 panels - Relative Quantification
Olink Target 96 provides targeted protein biomarker panels for analysing specific proteins related to a particular disease or biological function. They enable you to gain new insights into disease processes, improve disease detection and contribute to a better understanding of biology.
• Choose from 11 carefully designed panels available for human (10) and mouse (1)
Each panel is focused on a specific area of disease or key biology process
• Broad library of over 1100 established or exploratory protein biomarkers
• High quality data with exceptional readout specificity from our Proximity Extension Assay (PEA) technology
• Relative quantification
https://olink.com/products/olink-target-96
Due to the layout of the qPCR Dynamic arrays, 88 samples per analysis run (or multiples thereof) are recquired.
Available Panels:
Olink Target 96 Cardiometabolic (92)
Olink Target 96 Cardiovascular II (92)
Olink Target 96 Cardiovascular III (92)
Olink Target 96 Oncology II (92)
Olink Target 96 Immuno-Oncology (92)
Olink Target 96 Immune Response (92)
Olink Target 96 Neuro Exploratory (92)
Olink Target 96 Neurology (92)
Olink Target 96 Inflammation (92]
Olink Target 96 Metabolism (92)
Olink Target 96 Mouse Exploratory (92)
Olink Target 48 – Quantitative
– Olink® Target 48 Cytokine (45)
The Olink® Target 48 Cytokine panel offers carefully selected and validated assays covering 45 protein biomarkers important in inflammation and immune system regulation which are often involved in disorders like autoimmune diseases, neurological diseases, cardiovascular diseases, cancer, asthma, obesity, diabetes, psoriasis, and more.
Parallel measurement of all proteins is possible from only 1 µL of sample. In contrast to Target 96 products all Target 48 panels enable absolute quantification with output in pg/mL in addition to NPX values.
https://olink.com/products/olink-target-48
– Olink® Target 48 Immune Surveillance (44)
The Olink® Target 48 Immune Surveillance is a thoroughly validated human plasma-free panel that delivers quantification of 44 biomarkers in pg/mL, relevant for Immuno-oncology, Autoimmune Diseases, Transplantation, Vaccine, Allergy and Infection Immunity using only 1 μL sample.
Olink® Target 48 Immune Surveillance can be performed in parallel with Olink® Target 48 Cytokine to expand the coverage of biomarkers relevant for key therapeutic areas, delivering quantification of up to 89 proteins from just 2 μL of sample.
https://olink.com/products/olink-target-48
– Olink® Target 48 Mouse Cytokine (43)
With the Olink® Target 48 Mouse Cytokine panel it is possible to monitor longitudinal studies from murine immune response from only 1 µl of mouse sample. This reduces the variability of the study tremendously as the required animal use is minimized. 43 targets (relevant for Inflammation, Immune Response and Immuno-oncology) are measured in parallel, and the data will be provided in pg/ml (absolute quantification).
There is a 42% overlap between the Olink® Target 48 Cytokine and Olink® Target 48 Mouse Cytokine panels enabaling translational research on the same platform.
https://olink.com/products/olink-target-48-mouse
– Olink ® Target 48 Neurodegeneration (42)
The newest addition to the Olink ® Target 48 platforms empowers neurodegeneration research towards translational insights. Designed in partnership with field experts, biobanks, and publications to prioritize markers with translational potential, Target 48 Neurodegeneration panel covers mechanisms critical to interrogate disease progression and further understanding of leading neuro disorders such as Alzheimer’s, Parkinsons, and multiple sclerosis.
https://olink.com/products/olink-target-48/olink-target-48-neurodegeneration
Due to the layout of the qPCR Dynamic arrays, 40 samples per analysis run (or multiples thereof) are recquired.
Up to 30 plex – Olink FLEX – QUANTITATIVE and RELATIVE
Olink Flex is a flexible, made-to-order biomarker assay that can measure up to 30 human proteins across 40 samples simultaneously. Select and combine targets from a broad mix-and-match library of ~200 inflammation-related human proteins with 99% combinability. Consumes only 1 µL per sample. Results are reported in absolute quantification (pg/mL) and relative quantification (NPX).
Build the Olink Flex assay from scratch using a panel builder or select from curated template panels for cytokine storm, immuno-oncology, or interferon stimulation. Once the order is placed, analysis will be completed within 10 weeks. The minimum order is one assay for up to 30 proteins and 40 samples. Learn more
https://olink.com/products/olink-flex
Due to the layout of the qPCR Dynamic arrays, 40 samples per analysis run (or multiples thereof) are recquired.
Up to 21 plex – Olink FOCUS – QUANTITATIVE or RELATIVE
Olink Focus is a custom-made assay that provides an ideal solution for protein biomarker validation and beyond. Select and combine up to 21 proteins from the entire protein library of ~3000 human proteins. 144 or 160 samples can be analyzed simultaneously in one assay. Results are reported in absolute quantification (pg/mL) or relative quantification (NPX), and the validation level is customized based on your research needs.
The minimum order is a panel that enables the analysis of up to 21 proteins for 1000 samples. Olink experts will help you design your Olink Focus panel. Once the order is placed, the panel will be developed in less than 6 months. Learn more
SAMPLE TYPE – please inquire.
OLINK REVEAL – RELATIVE QUANTIFICATION
The newly released Olink Reveal panel spans over 1,000 proteins for broad coverage of the proteome and deep profiling of inflammation processes for disease research. This carefully curated library offers a multitude of robustly detectable markers with high proportion of cis-qQLTs for proteogenomics associations and is a useful tool for a variety of research questions.
- Broad proteome coverage: 100% top level pathways and 64% of all pathways in Reactome
- Deep profiling of inflammation: 537 inflammation proteins covering 96% of immune response pathways in Reactome
- Powerful for disease research: more than 850 proteins associated with disease
One Olink Reveal run supports 86 samples or multiples thereof.
OLINK® EXPLORE HT PANEL – relative quantification
The Olink® Explore HT panel is a high throughput biomarker discovery panel which targets more than 5.400 proteins (inflammatory proteins, tissue-enriched proteins, blood secreted proteins, drug target proteins, exploratory proteins). All protein assays are highly validated and with only 2 µl sample (plasma, serum) input the assay has a specificity of 99,5%. One Olink Explore HT run supports 172 samples or multiples thereof.
Sample sending and (pre-)processing
For serum and plasma samples there are no special requirements. For other sample types please see section „Sample Types“ and inquire for further instructions. In certain cases and upon request AIT can offer pre-processing like protein concentration measurements for standardization, sample concentration or dilution and aliquoting samples for specific project settings. These requirements should be defined and considered in advance.
Sample sending: sample-volume 30µl in 96 well plate (v-bottom – skirted) / tightly closed / sealed with aluminium foil suitable for -80°C, please spin down before freezing and send on dry ice.
SAMPLE LAYOUT and positions in the plate has to be defined according to the experiment descriptor layout and provided via email. Provide the reference number of the offer in the respective field.
Shipping address:
OLINK SERVICES
Molecular Diagnostics
Center for Health & Bioresources
AIT Austrian Institute of Technology GmbH
Giefinggasse 4| 1210 Vienna | Austria
– Optionally AIT can send back left-overs from samples at customer’s expensed – please let us know.
How it works
Service Request
Analysis request:
Please contact us via email to molecular-diagnostics@ait.ac.at – we will respond within one business day.
Shipping address:
AIT Austrian Institute of Technology GmbH
Center for Health & Bioresources
Competence Unit Molecular Diagnostics
OLINK® SERVICES
Giefinggasse 4
1210 Vienna
Austria