Extraction free miRNA/RNA analysis
AIT has long lasting experience in gene expression analysis and provides project and support for HTG technology based extraction free human mRNA and miRNA transcriptomic analysis from samples, in particular when RNA quality is impacted (e.g. archived FFPE tissue) and when only limited amounts are available. HTG technology enables RNA-extraction free analysis from FFPE tissue sections, serum, plasma from minimal sample amounts without any loss of sample material and bias due to extraction protocols. HTG technology has helped to manage analysis of critical samples and rescue projects which would have otherwise failed.
HTG technology enables
- Gene expression studies without the need for the previous extraction step, e.g. using a single 5μm thick FFPE tissue biopsy section.
- Analyse serum, plasma using minimal sample amounts without any loss of sample material and bias due to extraction protocols
- Sensitivity and dynamic range of NGS-based detection.
- Integrated data analysis suite for rapid data interpretation and publication.
Available panels: Oncological Immunology, Lymphomas, Total Transcriptome, Oncological Markers, and Breast.
AIT assists with all steps and helps you to carry out your analyses as simply as possible – from experimental planning and study design, pre-analytics processing and standardisation, analysing samples and bioinformatics data analysis, visualisation, and interpretation. In addition, AIT puts at your disposal a Service Provider authorized by HTG to carry out the studies without the need to purchase the equipment.
HTG panels provided
HTG Transcriptome Panel
• 19398 (mRNA) transcripts: profiling almost the entire transcriptome in a variety of samples
The HTG Transcriptome Panel (HTP) is expertly designed to provide extensive coverage of most human mRNA transcripts including isoforms. The panel can simultaneously interrogate 19,398 targets using Formalin Fixed Paraffin Embedded (FFPE), PAXgene and extracted RNA samples, generating data for up to 96 samples in less than three days. HTP utilizes our proprietary workflow and leverages the sensitivity and dynamic range of next-generation sequencing (NGS), allowing researchers to generate reliable results using limited sample amount.
For Research Use Only. Not for Use in Diagnostic Procedures.
Features and Benefits
- Expertly Curated Panel: Fast and easy to use solution to measure mRNA transcriptome
- Simplify your Transcriptome Analysis: Generate comprehensive mRNA profile with our streamlined workflow
- Extraction Free Workflow: Transcriptome analysis from limited amount and low quality RNA samples
- Compatible with common, biologically relevant sample types including FFPE, PAXgene and extracted RNA
- Simplified Data Analysis: Provides one click solution to visualization data and generate publication ready figures
Sample Requirements
HTG EdgeSeq miRNA Whole Transcriptome Assay
- 2083 human miRNA – analysis using a variety of samples
The HTG EdgeSeq miRNA Whole Transcriptome Assay (miRNA WTA) enables users to measure the expression of 2,083 human miRNA transcripts using next-generation sequencing (NGS). The HTG EdgeSeq chemistry is compatible with fixed tissue samples, biofluids, and cell lines. The miRNA WTA utilizes our automated HTG EdgeSeq system coupled with the sensitivity and dynamic range of NGS-based detection and can be performed in your lab using the HTG EdgeSeq system, from our VERI/O lab as a service, or at one of the Qualified Service Provider (QSP) sites.
For Research Use Only. Not for Use in Diagnostic Procedures.
Features and Benefits
- Simultaneous, quantitative detection of 2,083 human miRNA transcripts
- Profile circulating miRNA: obtain miRNA profiles from 15 µl of plasma or serum
- Extraction-free: reduce extraction-associated data bias and sample loss
- Gain insights faster: results in as little as 36 hours
- Simplified data analysis: raw numerical data output provided in Excel format
Sample Requirements
HTG EdgeSeq Oncology Biomarker Panel
- 2560 oncology-related genes – drug targets / signaling pathways
The HTG EdgeSeq Oncology Biomarker Panel (OBP) is a systems biology pathway analysis tool that profiles samples to identify therapeutic targets and drug response markers. The OBP assay utilizes our automated HTG EdgeSeq system coupled with the sensitivity and dynamic range of next-generation sequencing (NGS)-based detection and can be performed in your lab using the HTG EdgeSeq system, from our VERI/O lab as a service, or at one of the Qualified Service Provider (QSP) sites.
For Research Use Only. Not for Use in Diagnostic Procedures.
Features and Benefits
- Simultaneous, quantitative detection of 2,549 genes associated with tumor biology
- Extraction-free: reduce extraction-associated data bias and sample loss
- Reliable, reproducible performance from a single formalin-fixed, paraffin-embedded (FFPE) needle core biopsy slide
- Gain insights faster: results in as little as 36 hours
- Simplified data analysis: raw numerical data output provided in Excel format
Sample Requirements
HTG EdgeSeq Precision Immuno-Oncology Panel
- 1392 immuno-oncology related genes
The next-generation sequencing (NGS)-based HTG EdgeSeq Precision Immuno-Oncology Panel is designed to measure the immune response both inside the tumor and the surrounding microenvironment. HTG’s quantitative nuclease protection assay does not require nucleic acid extraction and is automated using the HTG EdgeSeq processor. By leveraging the high sensitivity and dynamic range of NGS instrumentation, this powerful tool interrogates 1,392 genes from a single section of formalin-fixed, paraffin-embedded (FFPE) tissue, extracted RNA, or PAXgene samples.
For Research Use Only. Not for Use in Diagnostic Procedures.
Features and Benefits
- Small sample input: test specific tumor microenvironment (TME) regions or needle core biopsies
- 1,392 genes focused on tumor/immune interaction: see a broader view of the immune response in solid and liquid tumors
- FFPE and PAXgene samples: immunophenotype lymphocytes from both the tumor and peripheral blood
- Tumor subtyping: categorize tumors based on previously defined molecular expression and immune response phenotypes
- Simplified data analysis: analyze the result without a complicated pipeline
Sample Requirements
HTG EdgeSeq Immune Response Panel
- 2002 immune pathway related genes
Measure 2,002 immune response mRNA targets in a single RNA extraction-free panel. The HTG EdgeSeq Immune Response Panel leverages the sensitivity and dynamic range of next-generation sequencing (NGS) to measure genes implicated in the immune response including response to pathogens and a variety of autoimmune diseases. The HTG EdgeSeq Immune Response Panel enables multiplex profiling from FFPE and PAXgene samples.
For Research Use Only. Not for Use in Diagnostic Procedures.
Features and Benefits
- Measure 2,002 transcripts in a single well: obtain a broad profile of genes associated with immune response to pathogen infection and autoimmune disease.
- Extraction-free chemistry: the simple workflow reduces extraction-associated data bias and sample loss
- Low sample input: reliable, reproducible performance from FFPE and PAXgene samples
- Simplified data analysis: analyze results in the HTG EdgeSeq Reveal data suite, not a complicated RNA-Seq pipeline
Sample Requirements
HTG EdgeSeq Pan B-Cell Lymphoma Panel
- 298 B-Cell Lymphoma relevant genes and DLBCL COO via DAC classifier
Measure gene expression of mRNA targets commonly associated with indolent and aggressive lymphomas to help identify lymphoma subgroups to elucidate the transcription response of disease state and drug therapy. With 298 gene targets, the HTG EdgeSeq Pan B-Cell Lymphoma Panel addresses the heterogeneity of B-Cell lymphomas, including published signatures and drug targets. Leveraging the sensitivity and dynamic range of next-generation sequencing (NGS), the HTG EdgeSeq Pan B-Cell Lymphoma Panel enables multiplex profiling from limited formalin-fixed, paraffin-embedded (FFPE) samples. The ability to work with small tissues is critical when considering that 10-15% of specimens in clinical practice do not qualify due to high sample requirements (PMID: 30248164). This extraction-free HTG EdgeSeq assay is automated on the HTG EdgeSeq system or available at our VERI/O lab as a service.
For Research Use Only. Not for Use in Diagnostic Procedures.
Features and Benefits
- Measure 298 transcripts in a single well: obtain a broad profile of genes associated with B-cell lymphomas from a single analysis
- Extraction-free chemistry: proprietary sample preparation workflow that reduces bias and sample loss associated with extracted RNA
- Low sample input: reliable results with as little as 6.0 mm2 tissue area per sample well
- Simplified data analysis: determine the Cell of Origin predicted probability of GCB for diffuse large B-cell lymphomas using the fully integrated HTG EdgeSeq Reveal data analysis pipeline
Sample Requirements
HTG EdgeSeq DLBCL Cell of Origin Assay
- ABC / GCB subtyping and profiling 92 lymphoma genes plus drug targets
The HTG EdgeSeq DLBCL Cell of Origin Assay characterizes diffuse large B-cell lymphoma (DLBCL) into the activated B-cell like (ABC), germinal center B-cell like (GCB) or unclassified subtype using a single 5 µm formalin-fixed, paraffin-embedded (FFPE) tissue section including core needle biopsies. The DLBCL Cell of Origin Assay utilizes our automated HTG EdgeSeq system coupled with the sensitivity and dynamic range of next-generation sequencing (NGS)-based detection, and can be performed in your lab using the
HTG EdgeSeq system, in our VERI/O lab as a service, or at one of the Qualified Service Provider (QSP) sites.
For Research Use Only. Not for Use in Diagnostic Procedures.
Features and Benefits
- Simultaneous, quantitative detection of 92 transcripts associated with B-cell lymphomas
- Classification and profiling from a single FFPE tissue section including needle core and other small biopsies
- Extraction-free: reduce extraction-associated data bias and sample loss
- Gain insights faster: results in as little as 36 hours
Sample Requirements
HTG EdgeSeq Mouse mRNA Tumor Response Panel
- 1659 murine immuno-oncology related mRNAs
Measure 1,659 mouse mRNA targets in one RNA extraction-free assay. The HTG EdgeSeq Mouse mRNA Tumor Response Panel leverages the sensitivity and dynamic range of next-generation sequencing (NGS) to measure genes implicated in preclinical mouse models of human disease.
Built around core signaling pathways and immune response mechanisms in oncology and other disease states, the HTG EdgeSeq Mouse mRNA Tumor Response Panel enables multiplex profiling from a variety of sample types, including a single section of formalin-fixed, paraffin-embedded (FFPE) tissue, cell lines, and extracted RNA samples. Applications include modeling oncogenesis, studying the immune response to mouse and patient-derived xenografts, and investigational therapeutic response studies. HTG’s extraction-free HTG EdgeSeq assays are automated on the HTG EdgeSeq system or can be run in HTG’s VERI/O service laboratory.
For Research Use Only. Not for Use in Diagnostic Procedures.
Features and Benefits
- Measure 1,659 transcripts in a single well: obtain a broad profile of genes associated with oncology, immunity, and inflammation
- Extraction-free chemistry: the simple workflow reduces extraction-associated data bias and sample loss
- Low sample input: reliable, reproducible performance from a single section of FFPE tissue
- Simplified data analysis: analyze results in the HTG EdgeSeq Reveal Data Suite, not a complicated RNASeq pipeline
Sample Requirements
MDP Panel
distributed by Durviz
MPD is a predictor of relapse combining Oncotype and Mammaprint output in one assay, for luminal breast cancer in early stages (stages I-II). With a concordance with Mammaprint and Oncotype above 80%. With the AIMS algorithm, the molecular subtype can also be provided (Luminal A, Luminal B, HER2, Triple negative, normal-like)
Sample Requirements
Sample types & Amounts – overview
Applications
Applications in Immuno-Oncology
Other Applications
How it Works
HTG Ressources
CONTENT
Analysis request:
Please contact us via email to molecular-diagnostics@ait.ac.at – we will respond within one business day.
Sample sending:
details will be clarified along answering your requests.
Sample layout and positions in the plate have to be defined according to the experiment descriptor layout and provided via email – please use the file “AIT-HTG-OrderSheet.xlsx” as template. Provide the reference number of the offer in the respective field.
Shipping address:
AIT Austrian Institute of Technology GmbH
Center for Health & Bioresources
Competence Unit Molecular Diagnostics
HTG SERVICES
Giefinggasse 4, 1210 Vienna
Austria